Coding

Part:BBa_K4212010:Design

Designed by: Youzi Bi   Group: iGEM22_Imperial_College_London   (2022-09-30)


GerAK_modified_chimera


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 992
    Illegal EcoRI site found at 1308
    Illegal PstI site found at 943
    Illegal PstI site found at 2762
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 992
    Illegal EcoRI site found at 1308
    Illegal PstI site found at 943
    Illegal PstI site found at 2762
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 992
    Illegal EcoRI site found at 1308
    Illegal BglII site found at 524
    Illegal XhoI site found at 2869
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 992
    Illegal EcoRI site found at 1308
    Illegal PstI site found at 943
    Illegal PstI site found at 2762
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 992
    Illegal EcoRI site found at 1308
    Illegal PstI site found at 943
    Illegal PstI site found at 2762
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 179


Design Notes

Combined GerAA, GerAC and GerKB into a single construct


Source

Synthetic construct using native coding sequences from GerA sub-units and GerK sub-units of B. subtilis.

References